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A6535
Sigma
Apyrase from potato
ATPase ≥200 units/mg protein, lyophilized powder
| Synonym: | Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase |
| CAS Number: | 9000-95-7 |
| Enzyme Commission (EC) Number: | 3.6.1.5 ( BRENDA | IUBMB ) |
| EC Number: | 232-569-8 |
| MDL number: | MFCD00130542 |
Description
| Application | At least two isoenzymes are found in different varieties of S. tuberosum:1,2 one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1). Reaction: ATP → ADP+Pi → AMP+2Pi. |
| Other Notes | This grade has a low ATPase/ADPase ratio. |
| Packaging | Sold on the basis of ATPase units. |
| Preparation Note | Derived from red potato |
| Unit Definition | One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C. |
| Physical form | Partially purified, lyophilized powder containing potassium succinate buffer salts. |
Properties
| type | Grade VII |
| form | lyophilized powder |
| secondary activity | ≥50 % of base activity ADPase |
| composition | Protein, 25-60% |
| EQP level | Premium |
| foreign activity | Acid Phosphatase ≤2% of base activity |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| Cited Reference | 1. Kettlun, A., et al. Phytochemistry 21, 551, (1982) |
| 2. Molnar, J. and Lorand, L. Arch. Biochem. Biophys. 93, 353, (1961) | |
| Merck | Merck 13,763 |
